Patterns and spatial distribution of sea turtle strandings in Alagoas, Brazil.

This study makes a descriptive analysis of necropsied sea turtles registered in the Biota Conservation Institute database between May 2018 and May 2022 on the coast of Alagoas, Brazil. During this period, 79 animals of four species were necropsied: 87.4 % (69) Chelonia mydas, 6.3 % (5) Caretta caretta, 3.8 % (3) Lepidochelys olivacea and 2.5 % (2) Eretmochelys imbricata. C. mydas was the most frequent species, mainly juvenile females. In 29.1 % (23/79) evidence of anthropogenic interactions was found (e.g., fishing net marks, plastic waste in the digestive tract, trauma from collisions with boats). Cutaneous tumors suggestive of fibropapillomatosis in 35.4 % (28/79), in C. mydas and E. imbricata, half were in an area of high eutrophication, close to the capital. Endoparasites were found in 46.8 % (37/79) individuals. Information on strandings in the region is essential for understanding the use of the area and the impacts to which these animals are exposed.

Coevolutionary Implications of Obligate Commensalism in Sea Turtles: the Case of the Genus Hyachelia Barnard, 1967 (Crustacea, Amphipoda).

Obligate commensalism in the marine environment and its evolutionary role are still poorly understood. Although sea turtles may serve as ideal substrates for epibionts, within amphipods, only the genus Hyachelia evolved in obligate commensalism with turtles. Here, we report a new host record for Hyachelia lowryi on the hawksbill turtle and describe a larger distribution of the genus in the Atlantic Ocean on green and loggerhead turtles. Hyachelia spp. were sampled from nesting sites of Caretta caretta and feeding grounds of Eretmochelys imbricata and Chelonia mydas along the Brazilian coast. Insights regarding the coevolution of this remarkable genus with its hosts based on molecular analyses are inferred based on mitochondrial (COI) and nuclear (18SrRNA) genes using new and previously available sequences from the infraorder Talitrida. Divergence times for Hyachelia are around the Cretaceous (~127.66 Mya), corresponding to an ancient origin and in agreement with modern green turtle (Chelonioidea) radiation. Later, diversification of Hyachelia species is dated at about 26 Mya, suggesting a coevolutionary association between amphipods and Carettini/Chelonini sea turtles.

Functional capacity and quality of life of older adults practitioners of câmbio: a cross-sectional study

Abstract Aim: This study aimed to evaluate the levels of functional capacity and quality of life in older adult practitioners of câmbio. Methods: This is a cross-sectional analytic study that evaluated men and women aged over 60 years, practitioners of câmbio in the Rio Grande do Sul, Brazil. The participants underwent a functional capacity assessment, composed of the sit- and stand-up and handgrip tests. In addition, quality of life was assessed through the WHOQOL-bref questionnaire. Results: Participants were 69 ± 6 years and had a body mass index of 27.9 ± 4.1 kg/m2. The participants practiced câmbio approximately 2.7 ± 1.2 times per week. Regarding the quality of life, results according to the domains of the questionnaire, it was observed that the participants presented values above 75% of the maximum possible. Regarding the performance in the sit- and stand-up test, participants had mean of 22 repetitions (95%CI: 20 to 23) and the average time for 5 repetitions was 7.1 seconds (6.8 to 7.5). In the grip strength test, participants had mean 35 kg (95%CI: 33.7 to 38.2). Conclusion: Older adult practitioners of câmbio presented satisfactory levels of quality of life and a good functional capacity.

Psychological Suffering, Medicalization and Women: Contributions in the Field of Public Health / Sofrimento Psicológico, Medicalização e Mulher: Contribuições no Campo da Saúde Pública / Angustia Psicológica, Medicalización y Mujeres: Contribuciones en el Campo de la Salud Pública

In recent years we have seen a growing psychological suffering in women attended in primary health care in developing countries. This paper aims to analyze psychological suffering in a small city in northeastern Brazil and its relationship with medicalization. We applied 202 SRQ-20 questionnaires in 3 Basic Health Units in the first research step, which were analyzed with statistical support. In the second step, four women were interviewed in the primary health care service and the data were analyzed from Institutional Ethnography perspective. The results demonstrated a high number of primary health care users in psychological suffering (47.02%). The data also points to a significant prevalence of women in psychological suffering, the use of medicines and the struggles in dealing with the difficulties of everyday life. The medicalization of psychological suffering appeared several times in this study through the invisibility of these sufferings under the blanket of biomedical and medicalizing discourse. Those facts can affect contemporary women and the contradictions of being a woman in a capitalist and patriarchal society. (AU)

Nos últimos anos temos visto um crescimento de sofrimento psicológico em mulheres atendidas na assistência de saúde primária em países em desenvolvimento. Este artigo teve como objetivo analisar o sofrimento psicológico em uma pequena cidade no Nordeste do Brasil e sua relação com a medicalização. Aplicamos 202 questionários SRQ-20 na primeira etapa da pesquisa em 3 unidades de saúde pública. Na segunda etapa, quatro mulheres foram entrevistadas e os dados foram analisados com o apoio da Etnografia Institucional. Os resultados demonstraram um alto número de usuários dessas unidades de saúde pública em sofrimento psicológico (47,02%). Esses dados também apontaram para uma prevalência significante de mulheres em sofrimento psicológico, o uso de medicamentos e o esforço em lidar com as dificuldades cotidianas. A medicalização do sofrimento psicológico apareceu diversas vezes nos relatos analisados neste estudo, evidenciando a invisibilidade desses sofrimentos através do discurso biomédico e medicalizante. Esses fatores desvelam as dificuldades em viver na contradição de ser mulher na contemporaneidade inserida numa sociedade que se revela capitalista e patriarcal. (AU)

En los últimos años, hemos visto un aumento de la angustia psicológica en las mujeres atendidas en la atención primaria de salud en los países en desarrollo. Este artículo tuvo como objetivo analizar el sufrimiento psicológico en un pequeño pueblo del noreste de Brasil y su relación con la medicalización. Aplicamos 202 cuestionarios SRQ-20 en la primera etapa de la investigación en 3 unidades de salud pública. En la segunda etapa, cuatro mujeres han sido entrevistadas y los datos fueron analizados con el apoyo de la Etnografía Institucional. Los resultados mostraron un alto número de usuarios de estas unidades de salud pública en problemas psicológicos (47.02%). Estos datos también apuntaron una prevalencia significativa en las mujeres con problemas psicológicos, el uso de medicamentos y el esfuerzo para hacer frente a las dificultades diarias. La medicalización del sufrimiento psicológico apareció varias veces en los informes analizados en este estudio, mostrando la invisibilidad de estos sufrimientos a través del discurso biomédico y medicalizante. Estos factores revelan las dificultades para vivir en la contradicción de ser mujer en los tiempos contemporáneos insertos en una sociedad que se revela capitalista y patriarcal. (AU)

Different infective forms trigger distinct lesions in the colon during experimental Chagas disease.

With the control of vectorial transmission of Chagas disease caused by metacyclic trypomastigotes (MT) in endemic countries, other pathways of infection have become important. The infection caused by blood trypomastigotes (BT) is relevant in places where the blood transfusion and organ transplantation are poorly controlled. This study aimed to evaluate immunopathogenic parameters in the colon during the acute and chronic phases of experimental infection in Swiss mice infected with BT or MT forms of VL-10 strain of Trypanosoma cruzi. We have found that animals infected with MT forms presented lower survival rate, and higher tissue parasitism in the acute phase of the disease, which may be associated with the exacerbated activation of the immune system with the production of pro-inflammatory cytokines even in the chronic phase of infection. Taken together, these results can also be associated to the maintenance of the inflammatory process in chronic phase and an earlier denervation of myenteric plexus in colon. These findings emphasized the importance of the inoculum source and the strain, once different forms of different strains seem to promote distinct diseases.

Antibacterial activity of honeys from Amazonian stingless bees of Melipona spp. and its effects on bacterial cell morphology.

BACKGROUND: Stingless bee honey has great therapeutic potential, especially as an antimicrobial agent. In the present study, we evaluated the in vitro antibacterial potential of honey from Melipona spp. with occurrence in Rio Branco-AC and Xapuri-AC from the Amazonian region. Samples were collected from the species Melipona eburnea, Melipona grandis, Melipona flavolineata and Melipona seminigra. The antibacterial activity of the honey samples was tested against standard Gram-positive and Gram-negative bacteria and two strains isolated from bovine mastitis. RESULTS: In the agar diffusion assay, we observed antibacterial activity for the four honeys against the tested strains. The honey from M. flavolineata showed a minimmum inhibitory concentration (MIC) lower than 3.12% (v/v). The minimum bactericidal concentration values were larger than the MIC for most of the microorganisms tested. Scanning electron microscopy (SEM) showed the damaging effect of the honey of M. flavolineata on Staphylococcus aureus cells, as well as its inhibitory effect on cell division. CONCLUSION: The results of the present study demonstrate that the honey from stingless bees possesses in vitro antimicrobial activity against pathogenic bacteria. The effects observed by SEM show that honey from the Amazonian stingless bee M. flavolineata has promising therapeutic potential as a future antimicrobial agent. © 2020 Society of Chemical Industry.

Physical activity learning by medical students: the current picture in Brazil / Aprendizagem sobre atividade física por estudantes de Medicina: a situação hoje no Brasil

Abstract: Introduction: Physical activity is essential for preventing and treating many diseases. Although physicians are the most influential health professionals in advising their patients on the benefits of physical activity, most medical degree programs in Brazil do not seem to include physical activity topics in their curricula. Objective: This study aimed to investigate physical activity topics on active medical curricula in Brazil. Method: The research was conducted separately in April 2015 and February 2019 using a governmental resource, the e-MEC system, and search in internet databases. Data were split into categories according to the type of enrollment (mandatory or optional) of the courses that have subjects or thematic modules with topics on physical activity, type of activities (theoretical, practical, or theoretical-practical), and emphasis on the content (health, performance, or health and performance). Results: Of the 223 medical curricula compiled in 2015 and 286 in 2019, respectively, only 24 (10.8%) and 19 (6.7%) had at least one subject or thematic module concerning physical activity with emphasis on health. Conclusion: In Brazil, the number of undergraduate medical curricula covering physical activity topics is still small and suffered a reduction between 2015 and 2019, which should warn medical education institutions about the need to include physical activity longitudinally distributed contents, with theoretical and - if possible - practical approaches, and emphasis on health promotion and treatment of diseases, in mandatory curricular units.

Resumo: Introdução: A atividade física é essencial para prevenir e tratar muitas doenças. Embora os médicos sejam os profissionais de saúde que mais influenciam a orientação de seus pacientes sobre os benefícios da atividade física, a maioria dos programas de graduação em Medicina no Brasil parece não incluir tópicos sobre atividade física em seus currículos. Objetivo: Este estudo teve como objetivo investigar a presença de tópicos sobre atividade física nos currículos médicos ativos no Brasil. Método: A pesquisa foi realizada separadamente em abril de 2015 e fevereiro de 2019, utilizando um recurso governamental, o sistema e-MEC, e pesquisa em bancos de dados da internet. Os dados foram divididos em categorias, de acordo com a condição de matrícula (obrigatória ou opcional) dos cursos com disciplinas ou módulos temáticos contendo tópicos sobre atividade física, tipo de atividades (teóricas, práticas ou teórico-práticas) e ênfase no conteúdo (saúde, desempenho ou saúde e desempenho). Resultado: Dos 223 currículos médicos compilados em 2015 e 286 em 2019, respectivamente, apenas 24 (10,8%) e 19 (6,7%) apresentaram pelo menos uma disciplina ou módulo temático contendo tópico sobre atividade física com ênfase em saúde. Conclusão: No Brasil, o número de currículos de graduação em Medicina contemplando tópicos de atividade física ainda é pequeno e sofreu uma redução entre 2015 e 2019, o que deve servir de alerta para as instituições de ensino médico quanto à necessidade de inclusão de conteúdos longitudinalmente distribuídos sobre atividade física, com abordagens teórica e, se possível, prática, e com ênfase na promoção da saúde e no tratamento de doenças, em unidades curriculares obrigatórias.

Small Mammals as Carriers/Hosts of Leptospira spp. in the Western Amazon Forest.

Leptospira is a bacteria that causes leptospirosis and is transmitted through water, soil, or mud that is contaminated by the urine of infected animals. Although it is mainly associated with the urban environment, Leptospires also circulate in rural and wild environments. This study aimed to investigate the role of small mammals in leptospirosis epidemiology in the western Amazon, Brazil. In total, 103 animals from 23 species belonging to the orders Didelphimorphia and Rodentia were captured. Blood, kidney, and urine samples were collected and Microscopic Agglutination Test (MAT), lipL32 PCR, secY sequencing, and culturing were conducted. MAT was reactive on 1/15 sera, and no bacterial isolate was obtained. PCR yielded 44.7% positive samples from 16 species. Twenty samples were genetically characterized and identified as L. interrogans (n = 12), L. noguchii (n = 4), and L. santarosai (n = 4). No statistical association was found between the prevalence of infection by Leptospira spp. in small mammals within carrier/hosts species, orders, study area, and forest strata. Our results indicate a high prevalence of pathogenic Leptospira spp. in several rodent and marsupial species and report the first evidence of Leptospira spp. carrier/hosts in the Brazilian Western Amazon.

Low-cost, safe, and effective smoke evacuation device for surgical procedures in the COVID-19 age.

BACKGROUND: Smoke is generated by energy-based surgical instruments. The airborne by-products may have potential health implications. METHODS: We developed a simple way to use de conventional surgical evacuator coupled with de electrosurgical pen attached to a 14G bladder catheter for open surgery. It was used in ten prospective patients with breast cancer. RESULTS: We notice a high reduction in surgical smoke during all breast surgery. A questionnaire was used for all participants of the surgery to answer the impression that they had about the device. The subjective impression was that the surgical smoke in contact whit the surgical team was reduced by more than 95%. CONCLUSIONS: Surgical smoke is the gaseous by-product produced by heat-generating devices in various surgical procedures. Surgical smoke may contain chemicals particles, bacteria, and viruses that are harmful and increase the risk of infection for surgeons and all the team in the operation room due to long term exposure of smoke mainly in coronavirus disease 2019 age. The adapted device described is a very simple and cheaper way to use smoke evacuators attached with the monopolar electrosurgical pen to reduce smoke exposure to the surgical team worldwide.

Low-dose of benznidazole promotes therapeutic cure in experimental chronic Chagas' disease with absence of parasitism in blood, heart and colon.

Studies suggest that the dose of the standard benznidazole (BNZ) treatment regimen might be too high. We investigated the efficacy of BNZ 20 and 40 mg/kg/day compared with standard dose (100 mg/kg/day) to induce cure in mice infected with Trypanosoma cruzi Y strain in the acute and chronic phases of Chagas' disease. Our findings indicate that an experimental treatment with a BNZ low-dose (40 mg/kg/day) is similarly effective as the usual dose in the chronic mice model (100% of cure). In addition, the treatment in the chronic model of Chagas' disease presented better results than the acute model and colon appears to be a key tissue when it comes to evaluating treatment efficacy compared to blood and heart. Therefore, our data suggest the reconsideration of the current therapy, mainly in the chronic phase of the disease.

A inter-relação entre transtornos mentais comuns, gênero e velhice: uma reflexão teórica / The interrelationship between common mental disorders, gender and age: a theoretical reflection

Resumo Introdução Um dos problemas mais significativos de saúde pública na atualidade é a prevalência de transtornos mentais comuns (TMC) e o uso excessivo de medicamentos psicotrópicos. As mulheres são as que mais sofrem com os TMC e fazem uso de antidepressivos e ansiolíticos. Objetivo Desenvolver uma reflexão teórica sobre as inter-relações entre TMC, relações de gênero e velhice, bem como apontar algumas contribuições para a atenção primária à saúde mental. Método Foi realizada uma pesquisa de artigos no SciELO e Google Acadêmico. Dos 15 artigos encontrados, foram incluídos 13 sobre a temática e realizada uma análise crítica. Resultados Os TMC estão relacionados com as condições de vida e as iniquidades de gênero. Mulheres idosas, ao deixarem de desempenhar papéis sociais para os quais haviam se preparado e por muito tempo desempenharam, experimentam sintomas de TMC. Com a saída dos filhos de casa e as limitações físicas decorrentes do processo de envelhecer, muitas delas se encontram sem outros projetos de vida. Isso contribui para o agravamento dos sintomas. Conclusão A atenção primária à saúde precisa incluir discussões sobre velhice e gênero, além de incrementar ações de promoção em saúde mental em suas atividades cotidianas.

Abstract Background One of the most significant public health issues today is the prevalence of common mental disorders (CMD) and the overuse of psychotropic drugs. Women are suffering most from the CMD and, consequently, making more use of antidepressants and anti-anxiety drugs. Objective This study aims at making some theoretical considerations on the interrelations between CMD, gender relations, and aging. Method It is a theoretical reflection. An article search was conducted in SciELO and Google academic. Of the 15 articles found, 13 were included on the subject and a critical analysis was carried out. Results It is observed that CMD is related to living conditions and gender inequities. Older women, when they do not play social roles for which they had prepared themselves and which they have long played it, they experience symptoms of CMD. With the departure of children from home and the physical limitations resulting from the aging process, many of them find themselves without other life projects and this fact contributes to the worsening of symptoms. Conclusion Primary health care must include discussions of aging, gender and actions to promote mental health in their daily activities.

The erythrocyte membrane stability is associated with sleep time and social jetlag in shift workers.

The osmotic stability of the erythrocyte membrane (OSEM) has been associated with changes in lipid profile, blood glucose and blood pressure. Changes in these parameters are very frequent in shift workers, possibly because of the lack of synchronization of biological rhythms, which results in the social jetlag. However, the existence of association between OSEM and circadian misalignment has not been investigated in this population. Therefore, this study investigated whether shift work, sleep time and social jetlag (SJL) are associated with biochemical and hematological variables. A population consisting of 79 men working at night (n = 37) or during the day (n = 42), aged between 21 and 65 years and with a mean BMI of 27.56 ± 4.0 kg/m2, was investigated cross-sectionally in relation to sleep time, SJL, anthropometric (height, weight and waist circumference) and blood variables, with emphasis on the OSEM. SJL was calculated by the absolute difference between the midpoint of sleep on work and rest days. The Generalized Linear Model (GzLM) was used to investigate the existence of associations between SJL and average sleep time in relation to the analyzed variables. Workers without SJL presented lower baseline lysis values of erythrocytes in isotonic medium in relation to workers with SJL. In addition, workers who slept on average less than 6 hours had higher OSEM, and higher total and LDL-cholesterol in relation to those who slept more than 6 hours, regardless of the shift. It is possible that the association of sleep deprivation and SJL with erythrocyte membrane stability is mediated through changes in the lipid profile.

A systematic review on the distribution of Mycobacterium bovis infection among wildlife in the Americas.

The occurrence of Mycobacterium bovis infection in wildlife places at risk livestock, public health, and ecosystems that house endangered species. However, data on wild species that may act as possible reservoirs in the Americas are scarce. This systematic review analyses the available data on wildlife in the Americas regarding the infection by M. bovis. We searched articles published in indexed journals using the keywords: "Mycobacterium bovis," "wild," and "animals". After applying the keywords using online databases, during March and August of 2018, we found 12 articles which encompassed 15 species of wild animals, of which three consisted of wild ruminants. The evidence showed that M. bovis is present among the wild animals in the Americas. The methodological limitations for diagnosing M. bovis in wild animals are many, demanding the development of new and more precise tools. Furthermore, new researches are needed to elucidate the role of the wild animals in the epidemiology of M. bovis and its possible impact on production animals and public health.

Night workers have lower levels of antioxidant defenses and higher levels of oxidative stress damage when compared to day workers.

The effects of circadian misalignment and work shift on oxidative stress profile of shift workers have not been explored in the literature. The present study aimed to evaluate the role of shift work (day and night) and social jetlag - a measure of circadian misalignment - with oxidative stress markers. A cross-sectional study was performed with 79 men (21-65 years old, 27.56 ± 4.0 kg/m2) who worked the night shift (n = 37) or daytime (n = 42). The analyzed variables included anthropometric measures and determination of systemic levels of markers of oxidative damage and antioxidant defense. Social jetlag was calculated by the absolute difference between the mean sleep point on working and rest days. The night group presented higher systemic values of thiobarbituric acid reactive substances and hydrogen peroxide, and lower levels of nitrite, total antioxidant capacity, and catalase and superoxide dismutase activities in relation to the day group. However, social jetlag was not associated with oxidative stress-related biomarkers analyzed in the night group. These results suggest that the night worker has higher levels of oxidative stress damage and lower levels of antioxidant defenses, while social jetlag was not a possible responsible factor for this condition.

The role of the erythrocyte in the outcome of pregnancy with preeclampsia.

The objective of this study was to analyze the relationships of osmotic and mechanical stability of erythrocytes with anthropometric, biochemical, hematologic and hemodynamic variables in pregnant women with preeclampsia (PE). The studied population consisted of 20 normotensive patients and 16 patients with PE. Patients with PE presented worse gestational outcome, greater hematologic impairment, erythrocytes osmotically more stable in vitro, but in conditions of isotonicity with the in vivo medium, in addition to hyperflow in orbital territory, when compared to normotensive patients. The correlation analysis between anthropometric, hematologic and hemodynamic variables in patients with PE indicated that erythrocytes with lower volumes and lower levels of hemoglobin favor the occurrence of a better gestational outcome, because they are more stable and because they are associated with a decrease in the hemodynamic changes present in the disease. This should mean that the tendency to microcytosis, probably due to a mechanism of compensatory mechanical selection, is a desirable characteristic in the disease.

Ingestion of plastic marine litter by sea turtles in southern Brazil: abundance, characteristics and potential selectivity.

The ingestion of plastic marine litter (PML) by sea turtles is widespread and concerning, and the five species that occur in the southwestern Atlantic - green, loggerhead, olive ridley, leatherback and hawksbill - are vulnerable to this pollution. Here, we quantified and characterized PML ingested by these species in southern Brazil, and observed PML ingestion in 49 of 86 sampled individuals (~57.0%). Green turtles presented the highest rates and variety of ingested plastics, and such ingestion has been high at least since 1997. Omnivorous turtles presented higher PML ingestion than carnivorous ones. Loggerheads displayed a negative correlation between body size and number of ingested items. Green turtles ingested mostly flexible transparent and flexible/hard white plastics; loggerheads ate mainly flexible, hard and foam fragments, in white and black/brown colors. These results help us better understand PML ingestion by sea turtles, highlighting the seriousness of this threat and providing information for prevention and mitigation strategies.

Toxicological aspects of trihalomethanes: a systematic review.

Chlorine is considered the most used chemical agent for water disinfection worldwide. However, water chlorination can lead to by-product generation which can be toxic to humans. The present study aimed to perform a systematic review on the toxicity of trihalomethanes (THMs) through bioindicators of cytotoxicity, genotoxicity, and mutagenicity. The results showed that studies on the effects of THMs on DNA are a current research concern for evaluating the toxicity of the pure compounds and real samples involving several types including water for recreational use, reused water, and drinking water. THMs deleterious effects have been assessed using several biosystems, where the Ames test along with experimental animal models were the most cited. A wide range of THM concentrations have been tested. Nevertheless, DNA damage was demonstrated, highlighting the potential human health risk. Among the studied THMs, chloroform presented a different action mechanism when compared with brominated THMs, with the former being cytotoxic while brominated THMs (bromodichloromethane, bromoform, and dibromochloromethane) were cytotoxic, genotoxic, and mutagenic. The described evidence in this research highlights the relevance of this topic as a human health issue. Nevertheless, research aimed to represent THMs current exposure conditions in a more accurate way would be needed to understand the real impact on human health.

The urban and rural capybaras (Hydrochoerus hydrochaeris) as reservoir of Salmonella in the western Amazon, Brazil / As capivaras urbanas e rurais (Hydrochoerus hydrochaeris) como reservatório de Salmonella no oeste da Amazônia, Brasil

The capybara (Hydrochoerus hydrochaeris) is the largest rodent in the world. In the state of Acre, Brazil, populations of capybaras have been increasing significantly. The role of capybaras in the transmission of certain bacterial zoonotic infections is not well understood, including bacteria of the genus Salmonella. Salmonella spp. generally cause enteritis or septicemia in mammals, however many mammalian species can carry the bacteria asymptomatically and shed it in their feces. To better understand the possible role of capybaras as reservoirs of Salmonella spp., we conducted a study of Salmonella within fecal samples from capybara in Acre. In a convenience sample, 54 capybaras from two urban and two rural areas of Acre were captured and kept for three to four days for sampling. None of the animals were symptomatic of any intestinal illness. Three separate fecal samples were collected from each animal, during their stays in captivity. Each sample was cultured for the presence of Salmonella spp. at the bacteriology laboratory of the Veterinary College of the Federal University of Acre. Samples were seeded in tetrationate pre-enrichment broth and in pre-enrichment broth peptone. After a 24 hour of incubation all samples were streaked on MacConkey Agar (MC) and Salmonella-Shigella Agar (SS). Suggestive colonies were submitted to biochemical analysis. Salmonella compatible colonies according to biochemical profile were submitted to serotyping (Sorokit for Salmonella - Probac do Brasil). In addition, the first sample from each of the 54 capybara was tested for Salmonella spp. using PCR targeting gene hilA. Eight (5%) of the 162 samples examined by bacterial culture were positive for Salmonella spp., while four (7%) of the 54 examined by PCR were positive. From the eight positive animals on culture, five were from urban area and three from rural area. On PCR, only one positive animal was from urban area and four were from rural area. Overall, by either test, one of the 54 animals was positive. All samples were collected in free - living animals with no apparent clinical signs of salmonellosis, indicating the potential of capybara as reservoir on this ecosystem.(AU)

A capivara (Hydrochoerus hydrochaeris) é o maior roedor do mundo. No estado do Acre, Brasil, as populações de capivaras têm aumentado significativamente. O papel das capivaras na transmissão de certas infecções zoonóticas bacterianas não é bem compreendido, incluindo as bactérias do gênero Salmonella. Salmonella spp. geralmente causam enterite ou septicemia em mamíferos, porém muitas espécies de mamíferos podem carregar a bactéria de forma assintomática e eliminá-la em suas fezes. Para entender melhor o possível papel das capivaras como reservatórios de Salmonellaspp., realizamos um estudo para identificação de Salmonella spp. em amostras fecais de capivaras no Acre. Em uma amostra de conveniência, 54 capivaras de duas áreas urbanas e duas áreas rurais do Acre foram capturadas e mantidas por três a quatro dias para amostragem. Nenhum dos animais era sintomático de qualquer doença intestinal. Três amostras fecais foram coletadas de cada animal, durante sua permanência em cativeiro. Cada amostra foi cultivada para a presença de Salmonella spp. no Laboratório de Bacteriologia Veterinária da Universidade Federal do Acre. As amostras foram semeadas em caldo de pré-enriquecimento tetrationato e em peptona de caldo de pré-enriquecimento. Após 24 horas de incubação, todas as amostras foram semeadas em ágar MacConkey (MC) e ágar Salmonella-Shigella (SS). Colônias sugestivas foram submetidas a análises bioquímicas. Colônias compatíveis com Salmonella de acordo com o perfil bioquímico foram submetidas à sorotipagem (Sorokit para Salmonella - Probac do Brasil). Além disso, a primeira amostra de cada uma das 54 capivaras foi testada para Salmonella spp. usando PCR, visando gene hilA. Oito (5%) das 162 amostras examinadas por cultura bacteriana foram positivas para Salmonella spp. Enquanto quatro (7%) das 54 examinadas pela PCR foram positivas. Dos oito animais positivos em cultura, cinco eram de área urbana e três de área rural. Na PCR, apenas um animal positivo era de área urbana e quatro de área rural. Considerando o diagnóstico conjunto por ambos os testes, PCR e cultura, um animal foi considerado positivo. Todas as amostras foram coletadas em animais livres, sem sinais clínicos aparentes de salmonelose, indicando o potencial da capivara como reservatório nesse ecossistema.(AU)

Nanoencapsulation improved water solubility and color stability of carotenoids extracted from Cantaloupe melon (Cucumis melo L.).

Cantaloupe melon carotenoids were encapsulated in porcine gelatin, whey protein isolate and concentrate by emulsification O/W to evaluate which agent could promote an increase in water solubility, and color stability in yogurt. The average particle size obtained was 59.3 (2.60) nm-161.0 (27.30) nm. Encapsulated crude extract in porcine gelatin presented the smallest size and polydispersity index [0.4 (0.04)], and showed sphericity, smooth surface and low agglomeration in SEM. These results associated to the good chemical interaction between the raw materials shown by FTIR, justify the increase in water solubility [0.072 (0.007) mg.mL-1] compared to the crude extract [0.026 (0.003) mg.mL-1]. The yogurt added with this nanoencapsulate remained stable for 60 days, unlike the crude extract. The results show that the nanoencapsulation using gelatin increased water solubility and the potential of application of melon carotenoids in food as natural dyes.